To explore the regulatory
mechanism of miR- 122-3p in osteogenic differentiation of mouse
adipose-derived mesenchymal stem cells (mADSCs) . [Methods]
The mADSCs obtained form 6 male C57BL/6 mice were cultured in
osteoinductive medium, and the osteogenesis was evaluated. After
miR-122-3p, miR-NC, antimiR-122-3p, anti-NC genes were transfected into
the mADSCs with lentiviral vector, the expression of miR-122-3p and
osteogenic-specific genes including Ocn and Alp were assessed by
qRT-PCR, additionally, the expression of β-catenin was assessed by
Western blotting assay.[Results]
culture, the mADSCs presented osteogenic differentiation with positive
stains of ALP and alizarin red. After transfection, the overexpression
of miR- 122- 3p inhibited osteogenic differentiation of mADSCs, whereas
knockdown of miR-122-3p promoted osteogenic differentiation of mADSCs.
In addition, Western blotting assay revealed that overexpression of
miR-122-3p activated the Wnt/β-catenin signaling pathway, while
knock-down of miR-122-3p inhibited the Wnt/β-catenin signaling pathway. [Conclusion]
The miR-122-3p affects osteogenic differentiation of mADSCs by regulating Wnt/β-catenin signaling pathway.
miR- 122- 3p modulates osteogenic differentiation of mouse adipose mesenchymal stem cells through wnt / βcatenin signaling pathway LIN Hao, SUN Zhong-yi, ZHENG Yong, HU Mao-hua, WANG Hai-bin, SUN Hong-zhi, TIAN Ji-wei.
Orthop J Chin, 27(24): 2272-2277.