The Official journal of the China Association of Rehabilitation of Disabled Persons and the 88th Hospital of PLA

Composite Impact Factor:

0. 948 (2015)

Total citation frequency: 3868 (2015)


ISSN 1005-8478

CN 37-1247/R

miR- 122- 3p modulates osteogenic differentiation of mouse adipose mesenchymal stem cells through wnt / βcatenin signaling pathway

Author : LIN Hao, SUN Zhong-yi, ZHENG Yong, HU Mao-hua, WANG Hai-bin, SUN Hong-zhi, TIAN Ji-wei.     
Working unit : Department of Orthopedics, The Affiliated Nanjing Jiangbei People's Hospital, Nantong University, Nanjing 210048, China
Page : 2272-2277.
Key words : adipose mesenchyme stem cell, osteogenic differentiation, miR-122-3p, wnt/β catenin signaling pathway
Abstract: [Objective] To explore the regulatory mechanism of miR- 122-3p in osteogenic differentiation of mouse adipose-derived mesenchymal stem cells (mADSCs) . [Methods] The mADSCs obtained form 6 male C57BL/6 mice were cultured in osteoinductive medium, and the osteogenesis was evaluated. After miR-122-3p, miR-NC, antimiR-122-3p, anti-NC genes were transfected into the mADSCs with lentiviral vector, the expression of miR-122-3p and osteogenic-specific genes including Ocn and Alp were assessed by qRT-PCR, additionally, the expression of β-catenin was assessed by Western blotting assay.[Results] After osteoinductive culture, the mADSCs presented osteogenic differentiation with positive stains of ALP and alizarin red. After transfection, the overexpression of miR- 122- 3p inhibited osteogenic differentiation of mADSCs, whereas knockdown of miR-122-3p  promoted osteogenic differentiation of mADSCs. In addition, Western blotting assay revealed that overexpression of miR-122-3p activated the Wnt/β-catenin signaling pathway, while knock-down of miR-122-3p inhibited the Wnt/β-catenin signaling pathway. [Conclusion] The miR-122-3p affects osteogenic differentiation of mADSCs by regulating Wnt/β-catenin signaling pathway.
summary:
miR- 122- 3p modulates osteogenic differentiation of mouse adipose mesenchymal stem cells through wnt / βcatenin signaling pathway   
LIN Hao, SUN Zhong-yi, ZHENG Yong, HU Mao-hua, WANG Hai-bin, SUN Hong-zhi, TIAN Ji-wei.
Orthop J Chin, 27(24):   2272-2277.