The Official journal of the China Association of Rehabilitation of Disabled Persons and the 88th Hospital of PLA

Composite Impact Factor:

0. 948 (2015)

Total citation frequency: 3868 (2015)


ISSN 1005-8478

CN 37-1247/R

Effect of MGF and TGF-1 on adaptive change of gastrocnemius during tibia lengthening in rabbit

Author : CHENG Huan, YAO Jin-yan, ZHANG Hai-li, et al.     
Working unit : Department of Anatomy and Histology, Tianjin Medical University, Tianjin 300070, China
Page : 1982-1987
Key words : limb lengthening, rabbit, gastrocnemius, mechanogrowth factor, transforming growth factor-1

Abstract:

Objective

To analyze expression of mechano growth factor MGF) and transforming growth factor-1

TGF-1) in gastrocnemius during tibial lengthening, and explore their role and mechanism in the adaptive muscular change.

Methods

Forty-five adult Japanese white rabbits were randomly divided into 3 experimental groups with 15 animals in each group, associated with tibia lengthening of 1cm, 2cm and 3cm in the corresponding group, By contrast, other 2 rabbits used as the control, received only osteotomy without tibia lengthening. In the 3 experiment groups, gastrocnemius was harvested for immunohistochemical staining of MGF and TGF-1 at 5 time points in the mineralization phase, including 0, 7, 14, 28 and 56

days after lengthening finished, therefore each experimental group was re-divided into 5 subgroups with 3 rabbits in each sub⁃group.

Results

During the mineralization phase after bone lengthening, the levels of MGF and TGF-1 in the gastrocnemius varied regularly. At 0 day of the phase, the level of MGF was at the peak value, which is significantly higher than the control

group. As time elapsed, the MGF decreased gradually to the baseline level at 56 days. On other hand, the TGF-1 staining was negative at beginning of the phase, subsequently it increased gradually to the peak value at 14 days, after that it diminished to the baseline level at 56 days.

Conclusion

The MGF is at the highest level at just beginning of mineralization phase, however, decrease as time elapsed, by contrast, the TGF-1 is at the lowest level at beginning, increase and decrease over time, which might be related to the regeneration and repair after damage of stretched gastrocnemius.


summary:
Effect of MGF and TGF-1 on adaptive change of gastrocnemius during tibia lengthening in rabbit   
CHENG Huan, YAO Jin-yan, ZHANG Hai-li, et al.
Orthop J Chin,2017,25(21):   1982-1987