The Official journal of the China Association of Rehabilitation of Disabled Persons and the 88th Hospital of PLA

Composite Impact Factor:

0. 948 (2015)

Total citation frequency: 3868 (2015)


ISSN 1005-8478

CN 37-1247/R

Preparation of nano hydroxyapatite (HA) 2-hydroxyethyl methacrylate scaffold and its functional evaluation

Author : HOU Yang, SHI Jian-gang, YUAN Wen.     
Working unit : Department of Orthopaedic Surgery, Changzheng Hospital, Shanghai 200003, China
Page : 441-447
Key words : nano hydroxyapatite, artificial bone, bone morphogenetic protein 7 (BMP-7) , bone marrow mesenchymal stem cells (BMSCs)

 Abstract:

Objective

In vitro evaluation of the biological properties of the nano hydroxyapatite (HA)/2-hydroxyethyl methacrylate (nHA/pHEMA) scaffold and its effects on osteogenic differentiation of bone marrow mesenchymal stem cells (BM? SCs) transduced with bone morphogenetic protein 7 (BMP-7) .

Methods

The nHA was combined with Poly (2- hydroxyethyl methacrylate) or pHEMA to construct the nHA/pHEMA composite scaffold. Subsequently the BMSCs transduced with BMP-7 were seeded into the nHA/pHEMA scaffold. The MTT, fluorescence microscopy and scanning electron microscope were used to evaluate the biocompatibility and cytotoxicity of the nHA/pHEMA scaffold. The ALP activity and RT-PCR tests were performed to explore effects of BMP-7 on the BMSCs seeded on the scaffold. Finally, the unconfined compression test was conducted to evaluate biomechanical properties of the nHA/pHEMA scaffold.

Results

The MTT, fluorescence microscopy and scanning electron icroscope showed good growth and proliferation of BMSCs seeded on the nHA/pHEMA scaffold. There was no significant difference in cell proliferation between the BMP-7-BMSCs and BMSCs groups (P>0.05) . However, the ALP activities in BMP-7-BMSCs group were significantly higher than the BMSCs group at 7 and 14 days (P<0.05) . In addition, the RT-PCR indicated that expression levels of RUNX2, COLI, OPN and OCN in BMP-7-BMSCs group were significantly superior to the BMSCs group at 7 and 14 days of in vitro culture (P<0.05) . Furthermore, the biomechanical test demonstrated that there was no brittle fracture in the nHA/pHEMA scaffold under conditions of high compressive strength and strain.

Conclusion

The nHA/ pHEMA scaffold has excellent biocompatibility and biomechanical properties. The BMP-7 can significantly enhanced the osteogenic differentiation of BMSCs seeded on the nHA/ pHEMA scaffold. The BMP-7-BMSCs combined with nHA/ pHEMA scaffold can be used as a type of promising bone re?pair material to promote bone formation.


summary:
Preparation of nano hydroxyapatite (HA) 2-hydroxyethyl methacrylate scaffold and its functional evaluation   
HOU Yang, SHI Jian-gang, YUAN Wen.
Orthop J Chin,2018,26(5):   441-447