In vitro evaluation of the
biological properties of the nano hydroxyapatite (HA)/2-hydroxyethyl
methacrylate (nHA/pHEMA) scaffold and its effects on osteogenic
differentiation of bone marrow mesenchymal stem cells (BM? SCs)
transduced with bone morphogenetic protein 7 (BMP-7) .
The nHA was combined with Poly (2- hydroxyethyl methacrylate) or pHEMA
to construct the nHA/pHEMA composite scaffold. Subsequently the BMSCs
transduced with BMP-7 were seeded into the nHA/pHEMA scaffold. The MTT,
fluorescence microscopy and scanning electron microscope were used to
evaluate the biocompatibility and cytotoxicity of the nHA/pHEMA
scaffold. The ALP activity and RT-PCR tests were performed to explore
effects of BMP-7 on the BMSCs seeded on the scaffold. Finally, the
unconfined compression test was conducted to evaluate biomechanical
properties of the nHA/pHEMA scaffold.
MTT, fluorescence microscopy and scanning electron icroscope showed good
growth and proliferation of BMSCs seeded on the nHA/pHEMA scaffold.
There was no significant difference in cell proliferation between the
BMP-7-BMSCs and BMSCs groups (P>0.05) . However, the ALP activities
in BMP-7-BMSCs group were significantly higher than the BMSCs group at 7
and 14 days (P<0.05) . In addition, the RT-PCR indicated that
expression levels of RUNX2, COLI, OPN and OCN in BMP-7-BMSCs group were
significantly superior to the BMSCs group at 7 and 14 days of in vitro
culture (P<0.05) . Furthermore, the biomechanical test demonstrated
that there was no brittle fracture in the nHA/pHEMA scaffold under
conditions of high compressive strength and strain.
The nHA/ pHEMA scaffold has excellent biocompatibility and biomechanical properties. The BMP-7 can significantly enhanced the osteogenic differentiation of BMSCs seeded on the nHA/ pHEMA scaffold. The BMP-7-BMSCs combined with nHA/ pHEMA scaffold can be used as a type of promising bone re?pair material to promote bone formation.