The Official journal of the China Association of Rehabilitation of Disabled Persons and the 88th Hospital of PLA

Composite Impact Factor:

0. 948 (2015)

Total citation frequency: 3868 (2015)


ISSN 1005-8478

CN 37-1247/R

Role of 3D cell spheres constructed by microcarrier and mesenchymal stem cells on peripheral nerve regeneration in vitro

Author : LIU Ping1,2, PENG Jiang2, WANG Yu2, XU Wen-jing2, MENG Hao-ye2, DU Ya-nan3, HOU Ang-yang1, CHANGFeng4.     
Working unit : 1. Shanxi Medical University, Taiyuan 030001, China; 2. Orthopaedic Institute, General Hospital of PLA, Beijing 100853, China; 3. Qinghua University, Beijing 100084, China; 4. People's Hospital Affiliated to Shanxi Medical University, Taiyuan 030012, China
Page : 2277-2281
Key words : microcarrier, adipose- derived stem cell,3D cell culture, dorsal root ganglion culture
  Abstract: [Objective] To explore the role of three-dimensional (3D) cell spheres constructed by microcarrier and mesenchymal stem cells (MSCs) on peripheral nerve regeneration in vitro. [Methods] The MSCs, extracted and isolated from groin adipose tissue of new born SD rats in 48 hours, were cocultured with microcarrier to construct 3D cell spheres. In addition, the cells were isolated from dorsal root ganglion (DRG) of new born rats in 12 hours. According to the different culture conditions,the axonal regeneration of the DRG cells was compared among following 3 groups the simple culture of DRG cells, the coculture of DRG cells with 2D MSCs, and the coculture of DRG cells with the 3D cells spheres. [Results] At 4 days of culture, the length of regenerating neurites proved (341.66±9.01) μm in the simple DRG cells group, (395.33±24.9) μm in the coculture with 2D MScs group, and (487.00±5.00) μm in the coculture with 3D cell spheres group. The effect of 3D cell spheres was significantly superior to the 2D MSCs for improving axonal regeneration of DRG cells in vitro (P<0.05) . [Conclusion] The 3D cell spheres based on microcarrier has more obvious effect on the growth of neurites in the dorsal root ganglion, which might provide high quality seed cells for neural tissue engineering in treatment of nerve defects, and improve the capacity of nerve regeneration.
summary:
Role of 3D cell spheres constructed by microcarrier and mesenchymal stem cells on peripheral nerve regeneration in vitro   
LIU Ping1,2, PENG Jiang2, WANG Yu2, XU Wen-jing2, MENG Hao-ye2, DU Ya-nan3, HOU Ang-yang1, CHANGFeng4.
Orthop J Chin,2018,26(24):   2277-2281