The Official journal of the China Association of Rehabilitation of Disabled Persons and the 88th Hospital of PLA

Composite Impact Factor:

0. 948 (2015)

Total citation frequency: 3868 (2015)


ISSN 1005-8478

CN 37-1247/R

A study on the difference of basement membrane of femoral nerve branches in rats

Author : WEI Shuai1, CHENG Xiaoqing2, WANG Yu2, WANG Wei-shan1, DING Xiao1, HAN Gong-hai2, LIU Ping3, PENG Jiang1, 2, SHI Chen-hui1.     
Working unit : 1. Medical Schoolof Shihezi University, Shihezi 832000, China; 2. Institute of Orthopaedics, General Hospital of PLA, Beijing 100853, China; 3. Orthopeadic Centre, The First Affiliated Hospital, Shihezi 832000, China
Page : 2282-2289
Key words : femoral nerve, muscle branch, cutaneous branch, basement membrane, basal lamina tube, raman spectroscopy
Abstract: [Objective] To observe the differences in the structure and components of the basement membranes of the branches of the femoral nerves in rats, and providing evidence for the precise regeneration of tissue-engineered nerves for peripheral nerve injury. [Methods] Thirty healthy 8-week-old male Sprague-Dawley rats were randomly divided into 2 groups. The animals in the experimental group were harvested femoral nerve and its branches for chemical decellularization, while those in the control group were gathered fresh femoral nerves and their branches. The ultrastructure, histology, immunohistochemistry, and Raman spectroscopy of the samples were observed. [Results] Regarding to the ultrastructure, the thickness of the myelin sheath of the femoral muscle branch was significantly greater than that of the cutaneous branch in the fresh samples (1.28±0.41 μm versus 0.81±0.32 μm, P<0.05) , whereas the thickness of the basement membrane of the femoral muscle branch proved significantly greater than that of the femoral cutaneous branch in the control group (34.55±2.23 nm versus 31.86±3.47 nm, P 0.05) . Scanning electron microscopy showed that the samples in the experimental group treated with decellularization was oriented tubular structure without visible cell structure, while those in the control group had obviously split nerve fibers and cell structure. In term of histological staining and immunofluorescence, the proportion of positive acetylcholinesterase staining in the femoral muscle branches was larger, while the proportion of negative sites in the cutaneous branch was larger in fresh samples of the control group. By contrast, the nerve fibers in the experimental group showed some nonnuclear structure by HE staining whereas only the red laminin background with no bright blue cell nuclear structure presented by DAPI and Laminin staining in the experimental group. In addition, the Raman spectroscopy showed that no a difference in the peak position was  noticed between the muscle branch and the cutaneous branch after decellularization in the experimental group, and the main residual components were lipids, proteins and trace nucleic acids. [Conclusion] There are considerable differences in the basement membrane structure between the muscular branch and cutaneous branches of the rat femoral nerve, despite of similar characteristics in Raman spectroscopy.
summary:
A study on the difference of basement membrane of femoral nerve branches in rats   
WEI Shuai1, CHENG Xiaoqing2, WANG Yu2, WANG Wei-shan1, DING Xiao1, HAN Gong-hai2, LIU Ping3, PENG Jiang1, 2, SHI Chen-hui1.
Orthop J Chin,2018,26(24):   2282-2289